Glucose Conversion for Biobutanol Production from Fresh Chlorella sorokiniana via Direct Enzymatic Hydrolysis

Microalgae, which accumulate considerable carbohydrates, are a potential source of glucose for biofuel fermentation. In this study, we investigated the enzymatic hydrolysis efficiency wet microalgal biomass compared with freeze-dried and oven-dried biomasses, both without an acidic pretreatment. With dilute sulfuric acid pretreatment followed by amy (α-amylase amyloglucosidase) cellulase hydrolysis, approximately 95.4% was recovered; however, 88.5% released 2% (w/v) acid, indicates acids direct saccharification process. There were no differences in yields among three kinds materials. any pretreatment, 78.7% yield obtained, addition had significant effect on to glucose. Compared biomass, produced substantially higher yield, is possibly because cross-linked cells prevented accessibility enzymes. According results, fresh cell disruption can be directly used produce The hydrolysate successfully acetone–butanol–ethanol (ABE) fermentation, 7.2 g/L ABE, indicating application microalgae bioalcohol fuel fermentation